Multi-label Fluorescence Spectra
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A new experiment with fluorescent dyes has been carried on. A single hypercube of a sample marked with different fluorescent molecules has been obtained. In this way it has been possible to unambiguously discriminate the different fluorophores by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein and quantum dots emitting in the yellow-orange region demonstrates the potentiality of multi-labeled fluorescence microscopy without the use of multiple filter set or dispersive means.
Layout of the HSI device for fluorescence measurements
Result: Fluorescence Spectra
Result: Fluorescence Spectra
The spectra calculated for each well of the sample. QDOT is the spectrum relative to the Quantum dot solution, FLUO is the Fluorescein spectrum and CY1 to CY5 are the different cyanine dies. In the graph the spectra are amplitude normalized to 1 in order to compare them more easily.
Result: False colour image
Result: False colour image
False color image where each pixel is assigned to one of the reference spectra of the previus figure. Despite the wide dynamic range, nearly all the pixels of each well have been assigned unambiguously to the correct class
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